期刊
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
卷 11, 期 1, 页码 81-92出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2015.1946
关键词
Protein-Based Nanoparticles; Ferritin; In Vivo Melanoma-Targeting; Magnetic Resonance Imaging (MRI) and Spectroscopy (MRS); Confocal Microscopy
资金
- Italian Association for Cancer Research (AIRC), Milan (IT) [MFAG10545]
- Italian Ministry of Economy and Finance (CZ) [RVO 61388971]
- Fondazione Anna Villa e Felice Rusconi Fund (IT)
- ENI Czech Republic s.r.o. (CZ)
- Manghi Czech Republic s.r.o. Fund (CZ)
- Paul's Bohemia Trading s.r.o. (CZ)
- Torino-Praga Invest s.r.o (CZ)
- MIUR [TUFO PON01_02433]
- Italian Ministry of Health
Nanoparticle (NP)-based materials are promising agents for enhancing cancer diagnosis and treatment. Once functionalized for selective targeting of tumor-expressed molecules, they can specifically deliver drugs and diagnostic molecules inside tumor cells. In the present work, we evaluated the in vivo melanoma-targeting ability of a nanovector (HFt-MSH-PEG) based on human protein ferritin (HFt), functionalized with both melanoma-targeting melanoma stimulating hormone (alpha-MSH) and stabilizing poly(ethylene glycol) (PEG) molecules. Independent and complementary techniques, such as whole-specimen confocal microscopy and magnetic resonance imaging, were used to detect in vivo localization of NP constructs with suitable tracers (i.e., fluorophores or magnetic metals). Targeted HFt-MSH-PEG NPs accumulated persistently at the level of primary melanoma and with high selectivity with respect to other organs. Melanoma localization of untargeted HFt-PEG NPs, which lack the alpha-MSH moiety, was less pronounced. Furthermore, HFt-MSH-PEG NPs accumulated to a significantly lower extent and with a different distribution in a diverse type of tumor (TS/A adenocarcinoma), which does not express alpha-MSH receptors. Finally, in a spontaneous lung metastasis model, HFt-MSH-PEG NPs localized at the metastasis level as well. These results suggest that HFt-MSH-PEG NPs are suitable carriers for selective in vivo delivery of diagnostic or therapeutic agents to cutaneous melanoma.
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