期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
卷 1839, 期 11, 页码 1256-1272出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbagrm.2014.09.002
关键词
Angiogenesis; Colorectal cancer; Microvesicles; miR-1246; Smad signaling
资金
- Ministry of Education, Science, Sports, and Culture of Japan [24659157]
- Japan Society for the Promotion of Science
- Grants-in-Aid for Scientific Research [24659157, 26870243] Funding Source: KAKEN
Emerging studies on circulating microRNAs (miRNAs) or microvesicles (MVs) have shown the potential of them to be novel biomarkers and therapeutic targets for cancer. However, the biological roles of these miRNAs and MVs have not been validated yet. To determine the biological significance of MVs, we used human colorectal cancer cells as the MV donor and endothelial cells (HUVECs) as the MV recipient and demonstrated the transfer of colorectal cancer cell-derived MVs (CRC-MVs) to HUVECs and evaluated the roles of these MVs and their cargo in tumor angiogenesis. Consequently, the incubation of HUVECs with CRC-MVs promoted the proliferation, migration, and tube formation activities of these cells. Among the cargoes shuttled by the MVs, miR-1246 and TGF-beta were considered to be responsible for the pro-angiogenic function of MVs by activating Smad 1/5/8 signaling in the HUVECs. These results suggest that colorectal cancer cells secreted MVs to contribute to tumor angiogenesis. (C) 2014 Elsevier B.V. All rights reserved.
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