期刊
INTERNATIONAL IMMUNOLOGY
卷 13, 期 3, 页码 385-394出版社
OXFORD UNIV PRESS
DOI: 10.1093/intimm/13.3.385
关键词
dendritic cells; HLA-G; mice; T cells
类别
资金
- NHLBI NIH HHS [HL60137] Funding Source: Medline
- NIAID NIH HHS [AI44219, AI42247] Funding Source: Medline
The human MHC class Ib antigen HLA-G is thought to regulate maternal immune responses during pregnancy. Here we show that expression of HLA-G in transgenic mice diminished cellular immunity by inhibiting maturation of myelomonocytic cells into functional antigen-presenting cells (APC), Skin allografts applied to HLA-G transgenic mice survived longer and resultant T cell responses were less potent compared to control mice, T cells from HLA-G mice responded normally to allogeneic APC and immunohistological analyses of spleen revealed no marked abnormalities. However, spontaneous outgrowths of myeloid cells were observed when bone marrow or splenocytes from HLA-G mice were cultured in vitro, but functionally competent APC did not develop spontaneously in bone marrow cultures supplemented with granulocyte macrophage colony stimulating factor (GM-CSF). Addition of lipopolysaccharide (LPS) to GM-CSF-derived bone marrow cultures rescued APC maturation. Studies using HLA-G tetrameric reagents revealed that HLA-G-specific binding activity was associated with CD11c(+) myelomonocytic cells, while binding to lymphoid and NK cell subsets was undetectable, These data show that spontaneous maturation of functionally competent dendritic cells IDC) is compromised in HLA-G mice. We hypothesize that HLA-G inhibits maturation of DC via receptor-mediated interactions with myelomonocytic precursors, which render immature DC precursors unable to receive signals from activated T cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据