期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
卷 1799, 期 7, 页码 510-519出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbagrm.2010.03.001
关键词
Drosophila; p38b MAPK; DREF; Transcriptional regulation; Intestinal stem cell; Midgut
资金
- Korea Research Foundation [KRF-2008-0737-000]
- Pusan National University
The Drosophila midgut is an excellent model for evaluation of gene networks that regulate adult stem cell proliferation and differentiation. The Drosophila p38b (D-p386) gene has been shown to be involved in intestinal stem cell (ISC) proliferation and differentiation in the adult midgut Here, we report that D-p38b gene expression is regulated by DREF (DNA replication-related element binding factor) in the adult midgut. We have identified a DRE in the S'-flanking region of the D-p38b gene and showed that DREF could bind to this DRE via a gel mobility shift assay and a ChIP assay. Base-substitution mutations of the D-p386 promoter DRE and analyses of transformants carrying D-p38b-lacZ or D-p38b-DREmut-lacZ indicated that this DRE is required for the activity of the D-p386 gene promoter. Furthermore, by using the GAL4-UAS system, we showed that DREF regulates the activity of the D-p38b gene promoter in adult ISCs and progenitors. In addition, the D-p38b knockdown phenotypes in the midgut were rescued by DREF overexpression, suggesting a functional link between these two factors. Our results suggest that the D-p38b gene is regulated by the DREF pathway and that DREF is involved in the regulation of proliferation and differentiation of Drosophila ISCs and progenitors. (C) 2010 Elsevier B.V. All rights reserved.
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