期刊
BIOLOGICAL CHEMISTRY
卷 382, 期 3, 页码 483-485出版社
WALTER DE GRUYTER & CO
DOI: 10.1515/BC.2001.059
关键词
endocytosis; fluorescence correlation spectroscopy; immunofluorescence; microinjection; multivesicular body; phospholipid
Late endosomes accumulate internal membranes within the lumen of the organelle. These internal membranes are enriched in the late endosome specific phospholipid, lysobisphosphatidic acid [LBPA]. The organization of LBPA-rich membrane domains is not well characterized. Using an LBPA-specific monoclonal antibody (6C4), we show that these membrane domains are not accessible from the cytoplasm. Using fluorescence correlation spectroscopy, we also show that 6C4 only binds sonicated, but not intact, late endosomes, presumably reflecting the release of internal membranes upon endosome rupture.
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