期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1828, 期 9, 页码 2230-2237出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2013.05.033
关键词
Bacillomycin L; Iturin; Rhizoctonia solani Kuhn; Membrane permeabilization; Mechanism of action
资金
- National Natural Science Foundation of China [31172001]
- Special Fund for Agro-scientific Research in the Public Interest of China [201303014]
Bacillomycin L, a natural iturinic lipopeptide produced by Bacillus amyloliquefaciens, is characterized by strong antifungal activities against a variety of agronomically important filamentous fungi including Rhizoctonia solani Kuhn. Prior to this study, the role of membrane permeabilization in the antimicrobial activity of bacillomycin L against plant pathogenic fungi had not been investigated. To shed light on the mechanism of this antifungal activity, the permeabilization of R. solani hyphae by bacillomycin L was investigated and compared with that by amphotericin B, a polyene antibiotic which is thought to act primarily through membrane disruption. Our results derived from electron microscopy, various fluorescent techniques and gel retardation experiments revealed that the antifungal activity of bacillomycin L may be not solely a consequence of fungal membrane permeabilization, but related to the interaction of it with intracellular targets. Our findings provide more insights into the mode of action of bacillomycin Land other iturins, which could in turn help to develop new or improved antifungal formulations or result in novel strategies to prevent fungal spoilage. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.
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