期刊
BIOMEDICAL MICRODEVICES
卷 3, 期 1, 页码 35-44出版社
SPRINGER
DOI: 10.1023/A:1011473203133
关键词
actuator; BioMEMS; cantilever; colloidal gold; immunosensor; nanoparticles
资金
- U.S. Department of Energy, Environmental Management Program [DOE DE-FG07-98ER62718]
- DOE Basic Energy Sciences [DE-FG02-96ER14609]
- National Science Foundation [CHE-9320461]
Microfabricated cantilevers, similar to those commonly used in scanning probe microscopies, have recently become increasingly popular as transducers in chemical and biological sensors. Surface stress changes that accompany intermolecular interactions on the cantilever surfaces offer an attractive means to develop new generations of microfabricated sensors and actuators that respond directly to chemical stimuli. In the present study, we demonstrate that interfacial molecular recognition events can be converted into mechanical responses much more efficiently when quasi 3-dimensional interfaces with nano-size features are used. Some of the particularly useful approaches to creating such interfaces are surface immobilization of gold nano-spheres and dealloying of co-evaporated Au:Ag films. Preliminary evaluation of these nanostructured surfaces was performed by measuring mechanical stresses generated by receptor modified nano-structures and smooth gold surfaces in response to gas-phase hydrocarbon compounds. The most efficient chemi-mechanical transduction was achieved when the cantilevers were modified with 50 to 75 nm thick dealloyed gold nanostrutures. Cantilevers of this type were selected for liquid phase experiments. These cantilevers were found to undergo several micron deflections upon adsorption of protein A and biotin-labeled albumin on nanostructured gold surfaces. Additional micrometer scale movements of the cantilevers were observed upon interaction of the surface bound bioreceptors with, respectively, immunoglobulin G and avidin from the aqueous phase.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据