期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 387, 期 1, 页码 143-153出版社
ACADEMIC PRESS INC
DOI: 10.1006/abbi.2000.2245
关键词
ascorbic acid; dehydroascorbic acid; GSH; thioredoxin reductase; mitochondria
资金
- NIA NIH HHS [AG16236] Funding Source: Medline
- NIDDK NIH HHS [DK 26657] Funding Source: Medline
- NIEHS NIH HHS [ES02497] Funding Source: Medline
Mitochondria generate reactive oxygen species as by-products of oxidative metabolism. Since ascorbic acid can scavenge such destructive species, we studied the ability of mitochondria from rat liver and muscle to take up, recycle, and oxidize ascorbate. Freshly prepared mitochondria contain ascorbate, as do mitoplasts that lack the outer mitochondrial membrane. Both mitochondria and mitoplasts rapidly take up oxidized ascorbate as dehydroascorbic acid and reduce it to ascorbate. Ascorbate concentrations in mitochondria and mitoplasts rise into the low millimolar range during dehydroascorbic acid uptake, although uptake and reduction is opposed by ascorbate efflux. Mitochondrial dehydroascorbic acid reduction depends mainly on GSH, but mitochondrial thioredoxin reductase may also contribute. Reactive oxygen species generated within mitochondria oxidize ascorbate more readily than they do GSH and cu-tocopherol. These results show that mitochondria can recycle ascorbate, which in turn might help to prevent deleterious effects of oxidant stress in the organelle. (C) 2001 Academic Press.
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