期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1808, 期 10, 页码 2618-2627出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2011.06.008
关键词
Bacterial phospholipase; Docking; Cell membrane; Pathogenicity; Sphingomyelinase; Phospholipid
资金
- Spanish Ministerio de Ciencia e Innovacion [BFU2007-62062, BFU2008-01637/BMC]
- CSIC - Universidad de Costa Rica [2008CR0030]
- Basque Government
Clostridium perfringens phospholipase C (CpPLC), also called a-toxin, is the main virulence factor for gas gangrene in humans. The lipase activity serves the bacterium to generate lipid signals in the host eukaryotic cell, and ultimately to degrade the host cell membranes. Several previous reports indicated that CpPLC was specific for phosphatidylcholine and sphingomyelin. Molecular docking studies described in this paper predict favorable interactions of the CpPLC active site with other phospholipids, e.g. phosphatidylethanolamine, phosphatidylinositol and, to a lesser extent, phosphatidylglycerol. On the basis of these predictions, we have performed experimental studies showing a-toxin to degrade all the phospholipids mentioned above. The molecular docking data also provide an explanation for the observed lower activity of CpPCL on sphingomyelin as compared to the glycerophospholipids. (C) 2011 Elsevier B.V. All rights reserved.
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