期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1808, 期 10, 页码 2496-2500出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2011.07.007
关键词
Oxidative stress; Angiotensin receptor; Image correlation spectroscopy; Aggregation
资金
- Thailand Research Fund [PHD/0245/2548]
- National Center for Genetic Engineering and Biotechnology (BIOTEC)
- National Science and Technology Development Agency (NSTDA, Thailand) [3-2548]
- Office of the Higher Education Commission
- Mahidol University under the National Research Universities Initiative
Oxidative stress and hyper-functioning of angiotensin II receptor type I (AT(1)R) are commonly observed in hypertensive patients but the relationship between oxidative stress and AT(1)R function is still unclear. We investigated the effects of H2O2 treatment on A-FIR-mediated intracellular calcium [Ca2+](i); signaling and its cell surface distribution pattern in HEK cells stably expressing EGFP-tagged rat AT(1)R using image correlation spectroscopy (ICS). Following H2O2 treatment (50-800 mu M), [Ca2+](i) was significantly increased upon angiotensin II stimulation. Similarly ICS revealed a significant increase in degree of AT(1)R aggregation in H2O2 treated group during Ang II activation but no difference in cluster density compared with untreated control cells or those with N-acetyl cysteine pretreatment. Thus, oxidative stress-induced AT(1)R hyper-responsiveness can be attributed by an increase in cell surface receptor aggregation state, possibly stemming in part from oxidant-related increase receptor-receptor interactions. (C) 2011 Elsevier B.V. All rights reserved.
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