期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1808, 期 2, 页码 572-579出版社
ELSEVIER
DOI: 10.1016/j.bbamem.2010.08.004
关键词
SARS-CoV; ORF8a; Ion channel; Molecular dynamics; Bilayer recording
资金
- National Yang-Ming University
- Government of Taiwan
- National Science Council of Taiwan (NSC)
- Alexander von Humboldt-Foundation
ORF8a protein is 39 residues long and contains a single transmembrane domain. The protein is synthesized using solid phase peptide synthesis and reconstituted into artificial lipid bilayers that forms cation-selective ion channels with a main conductance level of 8.9 +/- 0.8 pS at elevated temperature (38.5 degrees C). Computational modeling studies including multi nanosecond molecular dynamics simulations in a hydrated POPC lipid bilayer are done with a 22 amino acid transmembrane helix to predict a putative homooligomeric helical bundle model. A structural model of a pentameric bundle is proposed with cysteines, serines and threonines facing the pore. (C) 2010 Elsevier B.V. All rights reserved.
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