Apoptosis induced by peste des petits ruminants virus in goat peripheral blood mononuclear cells

The ability of peste des petits ruminants virus (PPRV) to induce apoptosis in goat peripheral blood mononuclear cell (PBMC) culture was investigated. Goat PBMC were infected with PPRV and the infectivity was con firmed by cytopathic effect, demonstration of presence of infectious viral progeny and expression of viral antigens in the lymphocytes, cultured in vitro. Infected PBMC showed morphological features of apoptosis. DNA extracted from PPRV-infected cells displayed laddering pattern in agarose gel electrophoresis. Infected cells also showed significantly higher apoptotic indices measured by bisbenzimide staining than control cells. Electronmicrographs of PPRV-infected PBMC revealed features typical of apoptosis such as peripheral condensation of chromatin, blebbing of plasma membrane. fragmentation of nucleus and cell leading to formation of apoptotic bodies. Our results suggest that PPRV can induce apoptosis, in vitro, in goat lymphocytes. (C) 2001 Elsevier Science B.V. All rights reserved.