期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
卷 104, 期 7, 页码 1457-1464出版社
WILEY
DOI: 10.1002/jbm.b.33496
关键词
cyclodextrin; indocyanine green; fluorescence; inclusion complex; vascular imaging
资金
- US Department of Energy, Office of Basic Energy Sciences [DE-AC02-98CH10886]
As the only FDA-approved near-infrared fluorophore, indocyanine green (ICG) is commonly used to image vasculature in vivo. ICG degrades rapidly in solution, which limits its usefulness in certain applications, including time-sensitive surgical procedures. We propose formulations that address this shortcoming via complexation with -cyclodextrin derivatives (-CyD), which are known to create stabilizing inclusion complexes with hydrophobic molecules. Here, we complexed ICG with highly soluble methyl -CyD and FDA-approved sulfobutyl ether -CyD (Captisol((R))) in aqueous solution. We measured the fluorescence of the complexes over 24 h. We found that both CyD+ICG complexes exhibit sustained fluorescence increases of >2.0x versus ICG in water and >20.0x in PBS. Using transmission electron microscopy, we found evidence of reduced aggregation in complexes versus ICG alone. We thus conclude that this reduction in aggregation helps mitigate fluorescence autoquenching of CyD+ICG complexes compared in ICG alone. We also found that while ICG complexed with methyl -CyD severely reduced the viability of MRC-5 fibroblasts, ICG complexed with sulfobutyl ether -CyD had no effect on viability. These results represent an important first step toward enhancing the utility of aqueous ICG by reducing aggregation-dependent fluorescence degradation. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1457-1464, 2016.
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