期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1798, 期 5, 页码 986-994出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2009.12.003
关键词
Pulmonary surfactant dysfunction; Captive bubble surfactometer; Cholesterol; Methyl-beta-cyclodextrin; Bovine lipid extract surfactant; Atomic force microscopy
资金
- CIHR (Canadian Institute of Health Research)
- ACHE (Alberta Children's Hospital Foundation)
- Alberta Lung Association
Pulmonary surfactant, a defined mixture of lipids and proteins, imparts very low surface tension to the lung-air interface by forming an incompressible film. In acute respiratory distress syndrome and other respiratory conditions, this function is impaired by a number of factors, among which is an increase of cholesterol in surfactant. The current study shows in vitro that cholesterol can be extracted from surfactant and function subsequently restored to dysfunctional surfactant films in a dose-dependent manner by methyl-beta-cyclodextrin (M beta CD). Bovine lipid extract surfactant was supplemented with cholesterol to serve as a model of dysfunctional surfactant. Likewise, when cholesterol in a complex with M beta CD (water-soluble cholesterol) was added in aqueous solution, surfactant films were rendered dysfunctional. Atomic force microscopy showed recovery of function by M beta CD is accompanied by the re-establishment of the native film structure of a lipid monolayer with scattered areas of lipid bilayer stacks, whereas dysfunctional films lacked bilayers. The current study expands upon a recent perspective of surfactant inactivation in disease and suggests a potential treatment. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据