期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1788, 期 10, 页码 2124-2131出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2009.07.017
关键词
Coiled-coil; Fluorescence imaging; Membrane protein; Tag-probe labeling
资金
- MEXT, Japan
Instead of using reconstituted proteoliposomes, in situ investigations of membrane proteins in living cell membranes are important because the heterogeneous and dynamic nature of biomembranes significantly affects their behavior. Protein-specific labeling is a key technique for the detection of a target protein by fluorescence measurements, particularly fluorescence microscopy. However, conventional genetic fusion with fluorescent proteins has several shortcomings. Post-translational labeling methods using a genetically encodable tag and synthetic probes targeting to the tag can overcome these limitations. This review summarizes emerging tag-probe techniques for labeling specific membrane proteins and their applications, including endocytotic internalization, partitioning to specific membrane domains, interprotein interactions, and conformational changes. (C) 2009 Elsevier B.V. All rights reserved.
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