期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1788, 期 12, 页码 2541-2552出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2009.10.006
关键词
Confocal fluorescence microscopy; Lipid rafts; Ternary phase equilibria; Deuterium nuclear magnetic resonance; Liquid ordered phase; Domain area fraction
资金
- Natural Sciences and Engineering Research Council of Canada
- Canada Foundation
- Ontario Research Fund
The differential miscibility of membrane lipids is thought to be the basis for the formation of dynamic microdomain assemblies in cell membranes known as membrane rafts. Because of their relevance to the existence of rafts, there has been much interest in recent years in model membrane systems that display coexisting liquid ordered (I-o) and liquid disordered phases (I-d), such as the ternary mixture composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and cholesterol. Carefully equilibrating the samples at well controlled temperatures allows us to use a quantitative confocal fluorescence microscopy approach to measure the area fractions of coexisting fluid phases in DOPC/DPPC/cholesterol mixtures. We can then compare the behaviour of a large population of unilamellar vesicles with the domain fractions deduced from H-2 NMR experiments. The fluorescence results are established for the first time to be in quantitative agreement with those obtained using H-2 NMR spectroscopy within the two phase region of the phase diagram. We are also able to describe fine details of the phase separation and the approach to equilibrium not previously reported, in particular the existence of small spots of I-o phase at temperatures higher than that at which the samples display domain fluctuations. A better understanding of coexisting fluid phases in model systems will assist in interpreting the behaviour of rafts in more complex biological membranes. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据