期刊
PLANT PHYSIOLOGY
卷 125, 期 3, 页码 1314-1324出版社
AMER SOC PLANT PHYSIOLOGISTS
DOI: 10.1104/pp.125.3.1314
关键词
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Genes and cDNAs for starch-branching enzyme II (SBEII) have been isolated from libraries constructed from Aegiloys tauschii and wheat (Triticum nestivum) endosperm, respectively. ene class of genes has been termed wSBEII-DA1 and encodes the N terminus reported for an SBEII. from wheat endosperm. On the basis of phylogenetic comparisons with other branching enzyme sequences, wSBEII-DA1 is considered to be a member of the SEEIIa class. The wSBEII-DA1 gene consists of 22 exons with exons 4 to 21 being identical in length to the maize (Zea mays) SBEIIb gene, and the gene is located in the proximal region of the long arm of chromosome 2 at a locus designated sbe2a. RN,A encoding SBEIIa can be detected the endosperm from 6 d after flowering and is at its maximum level from 15 to 18 d after anthesis. Use of antibodies specific for SBEIIa demonstrated that this protein was present in both the soluble and granule bound fractions in developing wheat endosperm. We also report a cDNA sequence for SBEIIa that could arise by variant transcription/splicing. A second gene, termed wSBEII-DB1, was isolated and encodes an SBEII, which shows greater sequence identity with SBEIIb-type sequences than with SBEIIa-type sequences. Comparisons of SBEII gene structures among wheat, maize, and Arabidopsis indicate the lineage of the SBEII genes.
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