期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1837, 期 1, 页码 1-13出版社
ELSEVIER
DOI: 10.1016/j.bbabio.2013.08.001
关键词
F1Fo-ATP synthase peripheral-stalk; Dimeric mitochondrial complex V; Chlorophycean algae; Chlamydomonas reinhardtii; Polytomella sp.; Asa subunits
资金
- Consejo Nacional de Ciencia y Tecnologia (CONACyT) [146044]
- Le Fonds National de la Recherche Scientifique (FNRS) (Mexico Belgium) [B330/123/11]
- CONACyT, Mexico [128110]
- Direccion General de Asuntos del Personal Academico) (DGAPA-UNAM, Mexico) [IN203311-3]
- Belgian F.R.S.-FNRS [MIS F.4520, FRFC 2.4597]
- PEW Charitable Trust for his postdoctoral fellowship
- Biomedical Sciences Ph.D. program at UNAM [229474, 214946]
Mitochondrial F1Fo-ATP synthase of chlorophycean algae is a complex partially embedded in the inner mitochondrial membrane that is isolated as a highly stable dimer of 1600 kDa. It comprises 17 polypeptides, nine of which (subunits Asa1 to 9) are not present in classical mitochondrial ATP synthases and appear to be exclusive of the chlorophycean lineage. In particular, subunits Asa2, Asa4 and Asa7 seem to constitute a section of the peripheral stalk of the enzyme. Here, we over-expressed and purified subunits Asa2, Asa4 and Asa7 and the corresponding amino-terminal and carboxy-terminal halves of Asa4 and Asa7 in order to explore their interactions in vitro, using immunochemical techniques, blue native electrophoresis and affinity chromatography. Asa4 and Asa7 interact strongly, mainly through their carboxy-terminal halves. Asa2 interacts with both Asa7 and Asa4, and also with subunit or in the F-1 sector. The three Asa proteins form an Asa2/Asa4/Asa7 subcomplex. The entire Asa7 and the carboxy-terminal half of Asa4 seem to be instrumental in the interaction with Asa2. Based on these results and on computer-generated structural models of the three subunits, we propose a model for the Asa2/Asa4/ Asa7 subcomplex and for its disposition in the peripheral stalk of the algal ATP synthase. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据