4.5 Article

Comparison of the α and β isomeric forms of the detergent n-dodecyl-D-maltoside for solubilizing photosynthetic complexes from pea thylakoid membranes

期刊

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1817, 期 8, 页码 1506-1515

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ELSEVIER
DOI: 10.1016/j.bbabio.2011.11.001

关键词

n-dodecyl-alpha-D-maltoside; n-dodecyl-beta-D-maltoside; Thylakoid; Membrane solubilization

资金

  1. Regione Piemonte (PROESA) [DGR. 3628559 7/04/2008]
  2. European Commission (SOLHYDRO-MICS) [227192]

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Mild non-ionic detergents are indispensable in the isolation of intact integral membrane proteins and protein-complexes from biological membranes. Dodecylmaltoside (DM) belongs to this class of detergents being a glucoside-based surfactant with a bulky hydrophilic head group composed of two sugar rings and a non-charged alkyl glycoside chain. Two isomers of this molecule exist, differing only in the configuration of the alkyl chain around the anomeric center of the carbohydrate head group, axial in alpha-DM and equatorial in beta-DM. In this paper, we have investigated the solubilizing properties of alpha-DM and beta-DM on the isolation of photosynthetic complexes from pea thylakoids membranes maintaining their native architecture of stacked grana and stroma lamellae. Exposure of these stacked thylakoids to a single step treatment with increasing concentrations (5-100 mM) of alpha-DM or beta-DM resulted in a quick partial or complete solubilization of the membranes. Regardless of the isomeric form used: 1) at the lowest DM concentrations only a partial solubilization of thylakoids was achieved, giving rise to the release of mainly small protein complexes mixed with membrane fragments enriched in PSI from stroma lamellae; 2) at concentrations above 30 mM a complete solubilization occurred with the further release of high molecular weight protein complexes identified as dimeric PSII, PSI-LHCI and PSII-LHCII supercomplexes. However, at concentrations of detergent which fully solubilized the thylakoids, the alpha and beta isomeric forms of DM exerted a somewhat different solubilizing effect on the membranes: higher abundance of larger sized PSII-LHCII supercomplexes retaining a higher proportion of LHCII and lower amounts Of PSI-LHCI intermediates were observed in alpha-DM treated membranes, reflecting the mildness of alpha-DM compared with its isomer. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial. (C) 2011 Elsevier B.V. All rights reserved.

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