期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 17, 期 2, 页码 143-148出版社
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1016609815405
关键词
mutagens; ethyl methanesulphonate; Haematococcus pluvialis; diphenylamine; nicotine; compactin; salinity stress
Cultures of Haematococcus pluvialis were exposed to mutagens like u.v. and EMS (ethyl methanesulphonate). The results showed that the survival rate decreased with the increase in u.v. exposure time and increase in EMS concentration. These mutants were further screened using inhibitors of the carotenoid biosynthetic pathway viz. diphenylamine (15-90 muM), nicotine (160-320 muM) and compactin (1.5-3.0 muM). The mutants thus obtained showed early enhanced (2.2-3.2-fold) astaxanthin accumulation and also exhibited higher lycopene cyclase activity.
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