期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1807, 期 3, 页码 368-374出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2010.11.007
关键词
Thylakoid membrane; Photosystem II; Supercomplex; Electron microscopy; Cryo tomography
资金
- Netherlands Organization of Scientific Research NWO
The architecture of grana membranes from spinach chloroplasts was studied by cryo electron tomography. Tomographic reconstructions of ice-embedded isolated grana stacks enabled to resolve features of photosystem II (PSII) in the native membrane and to assign the absolute orientation of individual membranes of granal thylakoid discs. Averaging of 3D sub-volumes containing PSII complexes provided a 3D structure of the PSII complex at 40 angstrom resolution. Comparison with a recently proposed pseudo-atomic model of the PSII supercomplex revealed the presence of unknown protein densities right on top of 4 light harvesting complex II (LHCII) trimers at the lumenal side of the membrane. The positions of individual dimeric PSII cores within an entire membrane layer indicates that about 23% supercomplexes must be of smaller size than full C2S2M2 supercomplexes, to avoid overlap. (C) 2010 Elsevier B.V. All rights reserved.
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