期刊
JOURNAL OF NUTRITION
卷 131, 期 3, 页码 787-795出版社
OXFORD UNIV PRESS
DOI: 10.1093/jn/131.3.787
关键词
fructooligosaccharides; genistein; daidzein; rats; time-resolved fluoroimmunoassay
The influence of dietary fructooligosaccharides (FOS) on bioavailability of genistein and daidzein in rats was estimated by measuring their concentrations in plasma collected from three different veins and in urine after a single intragastric administration of isoflavone conjugates. Sprague-Dawley male rats (6 wk old, n = 22) were fed a purified control (AlN-93G) diet or a FOS diet (AlN-93G + 5% FOS) for 7 d. A single dose of soy isoflavone conjugates, i.e., 8.5 mg as genistein and 33 mg as daidzein/kg body, was administered via a stomach tube at d 5. Blood samples were collected after administration via catheters in the portal and central Veins and by puncture of the tail vein. The isoflavones in plasma and urine were analyzed by time-resolved fluoroimmunoassay. The genistein concentration in the portal blood increased rapidly, reaching a peak of 3.5 mu mol/L in both groups at 1 h after administration. The concentrations in the central and tail venous blood were approximately half of those in the portal blood. In the FOS-fed group, both genistein and daidzein remained detectable at 24 and 48 h in the tail venous plasma. The urinary excretion of both isoflavones in the 24- to 48-h period after administration was significantly higher in the FOS-fed group than in the control group. The difference between the portal and central veins indicated hepatic uptake, probably leading to conjugation of aglycones and excretion into bile. FOS modified the absorption and enterohepatic recirculation of isoflavones.
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