期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1797, 期 8, 页码 1470-1476出版社
ELSEVIER
DOI: 10.1016/j.bbabio.2010.04.008
关键词
Mitochondria; Reactive oxygen species; Brown adipose tissue; Uncoupling protein 1
资金
- Marie Curie Transfer of Knowledge [COMPIB MTKD-CT-2005-030005]
- Science Foundation Ireland [SFI 06/IN.1/B67]
We provide evidence that ablation or inhibition of, uncoupling protein 1 increases the rate of reactive oxygen containing species production by mitochondria from brown adipose tissue, no matter what electron transport chain substrate is used (succinate, glycerol-3-phosphate or pyruvate/malate). Consistent with these data are our observations that (a) the mitochondrial membrane potential is maximal when uncoupling protein 1 is ablated or inhibited and (b) oxygen consumption rates in mitochondria from uncoupling protein 1 knock-out mice, are significantly lower than those from wild-type mice, but equivalent to those from wildtype mice in the presence of GDP. In summary, we show that uncoupling protein 1 can affect reactive oxygen containing species production by isolated mitochondria from brown adipose tissue. (C) 2010 Elsevier B.V. All rights reserved.
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