期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1797, 期 8, 页码 1681-1686出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2010.06.004
关键词
NDH-1; Electron microscopy; Subunit labeling; Yellow fluorescent protein; Synechocystis 6803
资金
- Academy of Finland [118637]
- Academy of Finland (AKA) [118637, 118637] Funding Source: Academy of Finland (AKA)
Cyanobacterial NDH-1 is a multisubunit complex involved in proton translocation, cyclic electron flow around photosystem I and CO2 uptake. The function and location of several of its small subunits are unknown. In this work, the location of the small subunits NdhL, -M, -N, -O and CupS of Synechocystis 6803 NDH-1 was established by electron microscopy (EM) and single particle analysis. To perform this, the subunits were enlarged by fusion with the YFP protein. After classification of projections, the position of the YFP tag was revealed; all five subunits are integrated in the membrane domain. The results on NDH-1 demonstrate that a GFP tag can be revealed after data processing of EM data sets of moderate size, thus showing that this way of labeling is a fast and reliable way for subunit mapping in multisubunit complexes after partial purification. (C) 2010 Elsevier B.V. All rights reserved.
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