期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 387, 期 1, 页码 35-40出版社
ELSEVIER SCIENCE INC
DOI: 10.1006/abbi.2000.2224
关键词
plasma membrane; Zea mays L.; corn roots; lipids; free sterols; glucocerebrosides; hydroxy fatty acids; very long chain fatty acids; phospholipids; fluorescence polarization
Although the results of lipid analyses from several plant species have been available for many years a complete characterization of the corn root plasma membrane is still lacking. The present study provides a detailed analysis of individual lipids and a characterization of the membrane fluidity of corn (Zea mays L,) root plasma membranes isolated by phase-partitioning. Phospholipids (43.9 mol%), sterols (40.8 mol%), and sphingolipids in the form of glucocerebroside (6.8 mol%) constitute the major lipid classes. Stigmasterol (19.8 mol%), campesterol (13.0 mol%), phosphatidylcholine 15.8 mol%), and phosphatidylethanolamine (14.2 mol%) represent the most ubiquitous individual lipids. Hydroxy fatty acids make up 80.9 mol% and very long chain fatty acids are almost 78% of fatty acids in glucocerebroside. Hydroxy arachidic acid (20:0 h) and hydroxy lignoceric acid (24:0 h) are most prominent and glucocerebroside from corn root plasma membranes contains virtually no unsaturated fatty acids. Among the phospholipids only phosphatidylserine displayed a high proportion of very long chain fatty acids (e.g., behenic and lignoceric acid). Membrane fluidity was estimated by fluorescence anisotropy, Due to the high sterol content the plasma membrane of corn roots is relatively rigid, (C) 2001 Academic Press.
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