期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1797, 期 6-7, 页码 1260-1267出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2009.12.015
关键词
Inner mitochondrial membrane; IK(Ca); HeLa cells; Patch-clamp; Calcium; TRAM-34
资金
- Associazione Italiana per la Ricerca sul Cancro Funding Source: Custom
The mitochondrial intermediate-conductance Ca2+-activated K+ channel mtK(Ca)3.1 has recently been discovered in the HCT116 colon tumor-derived cell line, which expresses relatively high levels of this protein also in the plasma membrane. Electrophysiological recordings revealed that the channel can exhibit different conductance states and kinetic modes, which we tentatively ascribe to post-translational modifications. To verify whether the localization of this channel in mitochondria might be a peculiarity of these cells or a more widespread feature we have checked for the presence of mtK(Ca)3.1 in a few other cell lines using biochemical and electrophysiological approaches. It turned out to be present at least in some of the cells investigated. Functional assays explored the possibility that mtK(Ca)3.1 might be involved in cell proliferation or play a role similar to that of the Shaker-type K(V)1.3 channel in lymphocytes, which interacts with outer mitochondrial membrane-inserted Bax thereby promoting apoptosis (Szabo, I. et al., Proc. Natl. Acad Sci. USA 105 (2008) 14861-14866). A specific K(Ca)3.1 inhibitor however did not have any detectable effect on cell proliferation or death. (C) 2009 Elsevier B.V. All rights reserved.
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