Micro-thermography in millimeter-scale animals by using orally-dosed fluorescent nanoparticle thermosensors

We propose an instant micro-thermography method using a fluorescent-nanoparticle thermosensor capable of reporting temperature as the fluorescence intensity ratio of the temperature-sensitive dye to the reference. We demonstrate temperature mapping inside a fruit fly larva that was orally dosed with nanoparticle thermosensors.