Determination of torque generation from the power stroke of Escherichia coli F1-ATPase

The torque generated by the power stroke of Escherichia coli F-1-ATPase was determined as a function of the load from measurements of the velocity of the gamma-subunit obtained using a 0.25 mu s time resolution and direct measurements of the drag from 45 to 91 nm gold nanorods. This result was compared to values of torque calculated using four different drag models. Although the gamma-subunit was able to rotate with a 20x increase in viscosity, the transition time decreased from 0.4 ms to 5.26 ms. The torque was measured to be 63 +/- 8 pN nm, independent of the load on the enzyme. (c) 2008 Elsevier B.V. All rights reserved.