期刊
BIOMATERIALS
卷 22, 期 6, 页码 619-626出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0142-9612(00)00225-8
关键词
tissue engineering; cartilage; photopolymerization; hydrogels; poly(ethylene oxide)
资金
- NHLBI NIH HHS [R01 HL60456] Funding Source: Medline
The thickness of human articular cartilage has been reported to vary from less than 0.5 up to 7 mm. Hence, tissue engineered cartilage scaffolds should be able to span the thickness of native cartilage to fill defects of all shapes and sizes. In this study, we demonstrate the potential fur using photopolymerization technology to encapsulate chondrocytes in poly(ethylene oxide) hydrogels, which vary in thickness from 2 to 8 mm. Chondrocytes. encapsulated in an 8 mm thick, photocrosslinked hydrogel and cultured in vitro for 6 weeks, remained viable and produced cartilaginous tissue throughout the construct comparable to a 2 mm thick gel as seen both histologically and biochemically. In addition, the total collagen and glycosaminoglycan contents per wet weight of the 8 mm thick cell-polymer construct were 0.13 +/- 0.01 and 0.25 +/- 0.03%, respectively, and did not vary significantly as a function of spatial position in the construct. The histological evidence and the biochemical content were similar in all constructs of varying thickness. The results suggest that photocrosslinked hydrogels are promising scaffolds for tissue engineering cartilage as cell viability is readily maintained: uniform cell seeding is easy to achieve; and the biochemical content of the extracellular matrix is not compromised as the scaffold thickness is increased from 2 to 8 mm. (C) 2001 Elsevier Science Ltd. All rights reserved.
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