期刊
BIOCHEMISTRY-MOSCOW
卷 76, 期 1, 页码 49-60出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S000629791101007X
关键词
Saccharomyces cerevisiae; translesion synthesis; recombination; chromosome stability
资金
- NATO [CBP.NR.NRCLG 982734]
- RFBR [09-04-13778-ofi-ts]
- Russian Academy of Sciences
- NIH [ES011644, ES015869]
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [K22ES011644, R01ES015869] Funding Source: NIH RePORTER
We employed a genetic assay based on illegitimate hybridization of heterothallic Saccharomyces cerevisiae strains (the alpha-test) to analyze the consequences for genome stability of inactivating translesion synthesis (TLS) DNA polymerases. The alpha-test is the only assay that measures the frequency of different types of mutational changes (point mutations, recombination, chromosome or chromosome arm loss) and temporary changes in genetic material simultaneously. All these events are manifested as illegitimate hybridization and can be distinguished by genetic analysis of the hybrids and cytoductants. We studied the effect of Pol zeta, Pol eta, and Rev1 deficiency on the genome stability in the absence of genotoxic treatment and in UV-irradiated cells. We show that, in spite of the increased percent of accurately repaired primary lesions, chromosome fragility, rearrangements, and loss occur in the absence of Pol zeta and Pol eta Our findings contribute to further refinement of the current models of translesion synthesis and the organization of eukaryotic replication fork.
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