期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 123, 期 10, 页码 2388-2392出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja003381p
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资金
- NIGMS NIH HHS [GM30741] Funding Source: Medline
Transient UV resonance Raman measurements excited within the amide pi --> pi* transitions of a 21 unit alpha -helical peptide has for the first time determined a lower bound for the unfolding rate of the last alpha -helical turn to form a fully random coil peptide. A 3 ns T-jump is generated with 1.9 mum laser pulses, which are absorbed by water. Subsequent 3 ns 204 nm UV pulses excite the amide Raman spectra at delay times between 3 ns and 1 ms, to monitor the peptide conformational evolution. We find similar to 180 ns relaxation times which result in a rate constant of >5 x 10(6) s(-1) for unfolding of the last alpha -helical turn. Our data are inconsistent with slow alpha -helix nuclei melting.
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