期刊
ANALYTICAL BIOCHEMISTRY
卷 290, 期 2, 页码 245-250出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/abio.2000.4959
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The hemoflavoenzyme cellobiose dehydrogenase (CDH, EC 1.1.99.18) from Phanerochaete chrysosporium has been used in an amperometric redox polymer-based biosensor. Used in conjugation with a FIA. system this biosensor can replace colorimetric assays for measuring cellobiose liberated from cellulose in a series of cellulase-containing samples. The biosensor gave the same result as the Somogyi-Nelson method in a less time-consuming and laborious manner. The two methods showed about the same precision. (C) 2001 Academic Press.
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