期刊
CELLULAR IMMUNOLOGY
卷 208, 期 2, 页码 88-95出版社
ACADEMIC PRESS INC
DOI: 10.1006/cimm.2001.1765
关键词
i-IEL; SCF; IL-7; IL-15; spontaneous apoptosis; caspase activity
资金
- NIDDK NIH HHS [DK02445, DK56605-01] Funding Source: Medline
Intestinal intraepithelial lymphocytes (i-IEL) readily undergo spontaneous apoptosis in vitro through an unclear mechanism. Here we examined the relationship between caspases, which plays a major role in apoptosis, and IL-7 in the spontaneous apoptosis of i-IEL in vitro, We demonstrated that IL-7 and zVAD prevented the spontaneous apoptosis of i-IEL by approximately 50% and 25% respectively with no additive protection seen when both are used. IL-7 preferentially prevented the apoptosis of gamma delta i-IEL, while zVAD equally prevented the apoptosis of gamma delta and alpha beta i-IEL, Lastly, we demonstrated that the spontaneous apoptosis of i-IEL is associated with a marked increase in caspase activity. Caspase activity was completely inhibited by zVAD, but only slightly by IL-7, Overall these results suggest that two pathways lead to the spontaneous apoptosis of i-IEL, one which is caspase dependent and the other which is caspase independent. IL-7 appears to exert its effect on i-IEL undergoing spontaneous by partially inhibiting both apoptotic pathways. (C) 2001 Academic Press.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据