期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 193, 期 6, 页码 689-698出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.193.6.689
关键词
T cell development; IL-7; T precursor cells; lineage commitment; T cell receptor gene rearrangement
Two types of T cells, alpha beta and gamma delta, develop in vertebrates. How these two T cell lineages arise from a common thymic T progenitor is poorly understood. Differentiation of alpha beta lineage T cells requires the surrogate ct chain (pT alpha), which associates with the T cell receptor (TCR) beta chain to form the pre-TCR. gamma delta lineage development does not appear to involve an obligatory surrogate chain, but instead requires productive rearrangement and expression of both TCR gamma and delta genes. It has been proposed that the quality of signals transmitted by the pre-TCR and gamma delta TCR are distinct and that these instructive signals determine the lineage fate of an uncommitted progenitor cell. Here we show that the thymic T progenitor cells (CD25(+) CD44(+)c-kit(+)CD3(-)CD4(-)CD8(-) thymocytes, termed pro-T cells) from young adult mice that have yet to express TCRs can be subdivided based on interleukin 7 receptor (IL-7R) expression. These subsets exhibit differential potential to develop into ya versus ap lineage (CD4(+)CD8(+) cells) in the thymus. Upon intrathymic injection, IL-7R(neg-lo) pro-T cells generated a 13-fold higher ratio of alpha beta lineage to gamma delta lineage cells than did IL-7R(+) pro-T cells. Much of this difference was due to a fivefold greater potential of IL-7R(+) pro-T cells to develop into TCR-ys T cells. Evidence indicates that this biased developmental potential is not a result of enhanced TCR-gamma gene rearrangement/expression in IL-7R(+) pro-T cells. These results indicate that the pro-T cells are heterogeneous in developmental potential before TCR gene rearrangement and suggest that in some precursor cells the initial lineage commitment is independent of TCR-mediated signals.
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