期刊
BIOCHEMISTRY
卷 40, 期 11, 页码 3289-3294出版社
AMER CHEMICAL SOC
DOI: 10.1021/bi002729q
关键词
-
资金
- NIAID NIH HHS [AI-30162] Funding Source: Medline
EXoS is a bifunctional type III cytotoxin that is secreted by Pseudomonas aeruginosa. The N-terminal domain comprises a RhoGAP activity, while the C-terminal domain comprises a ADP-ribosyltransferase activity. Previous studies showed that ExoS ADP ribosylated Pas at Arg41 which interfered with the ability of Ras to interact with its guanine nucleotide exchange factor. Rap and Ras share considerable primary amino acid homology, including Arg41. In this study, we report that ExoS ADP ribosylates Rap1b at Arg41 and that ADP ribosylation of Arg41 inhibits the ability of C3G to stimulate guanine nucleotide exchange. The mechanism responsible for this inhibition is one in which ADP-ribosylated Rap binds inefficiently to C3G, relative to wild type Rap. This identifies a second member of the Pas GTPase subfamily that can be ADP ribosylated by ExoS and indicates that ExoS can inhibit both Pas and Rap signaling pathways in eukaryotic cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据