期刊
CARBOHYDRATE RESEARCH
卷 331, 期 2, 页码 149-161出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0008-6215(01)00028-3
关键词
sucrose 6-phosphate; 6 '-phospho-alpha-D-glucosyl-D-fructoses; Klebsiella pneumoniae; phospho-alpha-glucosidase; sucrose 6-phosphate hydrolase; Family 4
Not only sucrose but the five isomeric alpha -D-glucosyl-D-fructoses trehalulose, turanose, maltulose, leucrose, and palatinose are utilized by Klebsiella pneumoniae as energy sources for growth. thereby undergoing phosphorylation by a phosphoenolpyruvate-dependent phosphotransferase system uniformly at O-6 of the glucosyl moiety. Similarly, maltose, isomaltose, and maltitol, when exposed to these conditions, are phosphorylated regiospecifically at O-6 of their non-reducing glucose portion. The structures of these novel compounds have been established unequivocally by enzymatic analysis, acid hydrolysis, FAB negative-ion spectrometry, and H-1 and C-13 NMR spectroscopy. In cells of K. pneumoniae, hydrolysis of sucrose 6-phosphate is catalyzed by sucrose 6-phosphate hydrolase from Family 32 of the glycosylhydrolase superfamily. The five 6'-O-phosphorylated alpha -D-glucosyl-fructoses are hydrolyzed by an inducible (similar to 49-50 Kda) phospho-alpha -glucosidase from Family 4 of the glycosylhydrolase superfamily. (C) 1001 Elsevier Science Ltd. All rights reserved.
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