期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 282, 期 1, 页码 90-95出版社
ACADEMIC PRESS INC
DOI: 10.1006/bbrc.2001.4555
关键词
CD39; E-NTPDase; apyrase; ADP; release; binding site; mechanism
资金
- NHLBI NIH HHS [HL08893] Funding Source: Medline
- NIDDK NIH HHS [DK27626] Funding Source: Medline
A soluble form of CD39 was expressed and purified from High-Five insect cells. The soluble CD39 is a monomer with a molecular weight of 54,000. The k(cat) and K-m of the purified soluble CD39 were 4.6 s(-1) and 12 muM for ATP and 1.3 s(-1) and 7 muM for ADP as substrates, respectively. One nucleotide binding site was detected on the monomer only in the presence of Ca2+. In contrast to the membrane bound CD39, soluble CD39 released ADP as an intermediate during ATP hydrolysis, as did the soluble potato apyrase, (C) 2001 Academic Press.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据