期刊
JOURNAL OF CHROMATOGRAPHY B
卷 753, 期 1, 页码 101-113出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(00)00424-2
关键词
human serum albumin
Chiral stationary phases obtained by immobilization of HSA on [C8] and [C18] reversed-phases and on poly(1-vinylimidazole)-coated silica were tested to resolve DL-tryptophan, N-benzoyl-DL-phenylalanine, RS-oxazepam and RS-warfarin racemic mixtures. Parameters of enantioselectivity measured in HPLC are correlated to structural and solvation states for adsorbed HSA, evaluated by FTIR spectroscopy. HSA immobilized on [PVI]-anion-exchangers is highly selective. HSA molecules are not self-associated, only unfolded for a small hydrophobic helix. The HSA-coated reversed-phases have a lower selectivity. Unfolding is larger but the indole-benzodiazepine chiral site is preserved and remains accessible. (C) 2001 Elsevier Science B.V. All rights reserved.
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