期刊
PROTEIN SCIENCE
卷 10, 期 4, 页码 887-891出版社
WILEY
DOI: 10.1110/ps.42301
关键词
polyglutamine; disaggregation; nucleation-dependent aggregation; seed; Huntington's disease
A method is described for dissolving and disaggregating chemically synthesized polyglutamine peptides. Polyglutamine peptides longer than about Q(20) have been reported to be insoluble in water, but dissolution in - and evaporation from - a mixture of trifluoroacetic acid and hexafluoroisopropanol converts polyglutamine peptides up to at least Q(44) to a form readily soluble in aqueous buffers. This procedure also has a dramatic effect on peptides which appear to be completely soluble in water, by removing traces of aggregate that seed aggregation. The protocol makes possible solution studies-including in vitro aggregation experiments-on polyglutamine peptides with repeat lengths associated with increased risk of Huntington's Disease and other expanded CAG repeat diseases. It may also be useful in conducting reproducible, quantitative aggregation studies on other polypeptides.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据