期刊
ENDOCRINE
卷 14, 期 3, 页码 311-323出版社
HUMANA PRESS INC
DOI: 10.1385/ENDO:14:3:311
关键词
endochondral ossification; 1 alpha,25-dihydroxyvitamin D-3; 24R,25-dihydroxyvitamin D-3; metalloproteinase; tissue inhibitor of metalloproteinases
资金
- NIAMS NIH HHS [AR-08662] Funding Source: Medline
- NIDCR NIH HHS [DE-08603, DE-05937] Funding Source: Medline
Recent studies indicate that 1 alpha ,25-dihydroxyvitamin D-3 (1(alpha),25[OH](2)D-3) and 24R,25-dihydroxyvitamim D-3 (24R,25[OH](2)D-3) differentially regulate proliferation, differentiation, and matrix synthesis of growth plate chondrocytes. To determine whether both metabolites play the same or different roles in vivo, we used the vitamin D-deficient rat as a model. Rickets was induced and then reversed by administering a single dose of ergocalciferol, 1 alpha ,25 (OH)(2)D-3, or 24R,25 (OH)(2)D-3 and euthanizing the animals after 4, 24, 48, or 72 h. Growth plates were either processed for histology and histomorphometry or extracted with buffered guanidine-HCl. Neutral metalloproteinase activity in the extracts was measured by use of aggrecan-containing beads, and collagenase activity was determined by use of radioactive type I collagen. The levels of tissue inhibitor of metalloproteinases (TIMP) and plasminogen activator were also determined. The morphology of the growth plate varied as a function of treatment. While 24R,25(OH)(2)D-3 appeared to affect cell maturation and 1 alpha ,25(OH)(2)D-3 appeared to affect terminal differentiation and calcification, response to ergocalciferol was indicative of the combined responses to the individual metabolites. Enzyme activity was regulated in a differential manner. Treatment with ergocalciferol produced a rapid decline in both neutral metalloproteinase and collagenase activities that was statistically significant by 4 h. By contrast, 1 alpha ,25(OH)(2)D-3 had no effect on neutral metalloproteinase activity but caused a significant decrease in both active and total collagenase activity by 4 h, while 24R,25(OH)(2)D-3 decreased neutral metailoproteinase activity by 48 h and had no effect on collagenase activity. Ergocalciferol had no effect on TIMP levels at any time examined, whereas 1 alpha ,25(OH)(2)D-3 caused an increase at 48 and 72 h and 24R,25(OH)(2)D-3 completely blocked TIMP production at 4 and 24 h. By contrast, plasminogen activator activity by ergocalciferol was decreased at 4 h, increased by 1 alpha ,25(OH)(2)D-3 at 4 and 24 h, and decreased by 24R,25(OH)(2)D-3 at all time points examined. These in vivo results confirm our previous cell culture observations showing that growth plate chondrocytes are differentially regulated by 1 alpha ,25(OH)(2)D-3 and 24R,25(OH)(2)D-3. Moreover, they show definitively that these two vitamin D metabolites play distinct roles not only in regulating neutral metalloproteinase and collagenase activities in growth plate cartilage but in cell maturation and calcification of this tissue in vivo.
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