期刊
FEMS MICROBIOLOGY LETTERS
卷 197, 期 1, 页码 99-103出版社
ELSEVIER SCIENCE BV
DOI: 10.1111/j.1574-6968.2001.tb10589.x
关键词
Bacteroides thetaiotaomicron; gastrointestinal tract; glucosinolate; sinigrin; allyl isothiocyanate; gnotobiology
类别
A human digestive strain of Bacteroides thetaiotaomicron was tested for its ability to metabolise sinigrin, a glucosinolate commonly found in Brassica vegetables. Gnotobiotic rats harbouring the bacterial strain were orally dosed with 50 mu mol sinigrin. HPLC analysis of the digestive contents showed that sinigrin was degraded in the large bowel, where B. thetaiotaomicron was established at a high level. Concurrently, a hydrolysis product of sinigrin, allyl. isothiocyanate, was identified by GC-MS analysis, following headspace solid-phase microextraction of the digestive contents;its production peaked at ca. 200 nmol, 12 h after dosing. This is the first study to demonstrate in vivo the involvement of a human colonic predominant bacterium in the bioconversion of a dietary glucosinolate to a potentially anticarcinogenic isothiocyanate. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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