Structural Basis for Assembly of the MnIV/FeIII Cofactor in the Class Ic Ribonucleotide Reductase from Chlamydia trachomatis

The class Ic ribonucleotide reductase (RNR) from Chlamydia trachomatis (CO employs a Mn-IV/Fe-III cofactor in each monomer of its beta(2) subunit to initiate nucleotide reduction. The cofactor forms by reaction of Mn-II/Fe-II-beta(2) with O-2. Previously, in vitro cofactor assembly from apo beta(2) and or divalent metal ions produced a mixture of two forms, with Mn eat site 1 (Mn-IV/Fe-III) or site 2 (Fe-III/Mn-IV), of which the more active Mn-IV/Fe-III product predominates: Here we have addressed the basis for metal site selectivity by determining X-ray crystal structures of apo, Mn-II, and Mn-II/Fe-II complexes of Ct beta(2). A structure obtained anaerobically with equimolar Mn-II, Fe-II, and apoprotein reveals exclusive incorporation of Mn-II at site 1 and Fe-II at site 2, in contrast to the more modest site selectivity achieved previously. Site specificity is controlled thermodynamically by the apoprotein structure, as only minor adjustments of ligands occur upon metal binding. Additional structures imply that, by itself, Mn-II binds in either site. Together, the structures are consistent with a model for in vitro cofactor assembly in which Fe-II specificity for site 2 drives assembly of the appropriately configured heterobimetallic center, provided that Fe-II is substoichiometric. This model suggests that use of a Mn-IV/Fe-III cofactor in vivo could be an adaptation to Fe-II limitation. A 1.8 angstrom resolution model of the Mn-II/Fe-II-beta(2) complex reveals additional structural determinants for activation of the cofactor, including a proposed site for side-on (eta(2)) addition of O-2 to Fe-II and a short (3.2 angstrom) Mn-II-Fe-II interionic distance, promoting formation of the Mn-IV/Fe-IV activation intermediate.