期刊
BIOCHEMISTRY
卷 51, 期 42, 页码 8321-8323出版社
AMER CHEMICAL SOC
DOI: 10.1021/bi300471d
关键词
-
资金
- W. M. Keck Foundation
- John S. Dunn, Sr., Foundation
By combining pseudorandom bead-based aptamer libraries with conjugation chemistry, we have created next-generation aptamers, X-aptamers (XAs). Several X-ligands can be added in a directed or random fashion to the aptamers to further enhance their binding affinities for the target proteins. Here we describe the addition of a drug (N-acetyl-2,3-dehydro-2-deoxyneuraminic acid), demonstrated to bind to CD44-HABD, to a complete monothioate backbone-substituted aptamer to increase its binding affinity for the target protein by up to 23-fold, while increasing the drug's level of binding 1-million fold.
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