期刊
JOURNAL OF VIROLOGICAL METHODS
卷 93, 期 1-2, 页码 49-56出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0166-0934(00)00296-2
关键词
recombinant swinepox virus; thymidine kinase; CSFV; E2
To explore the swinepox virus (SPV) as a potential live vector for immunization. a vector was developed for the construction of a recombinant SPV carrying foreign genes. In this system, a foreign gene placed under the strong vaccinia virus promoter P-11 can be inserted into the viral thymidine kinase (TK) gene, and the recombinant virus can be isolated in a non-selective medium by the co-expression of E. coli lacZ gene. Compared with the wild type virus, the TK-recombinant SPV showed a modest level of attenuation in porcine cells while more attenuation was observed in monkey or human cells. Using this system, a recombinant virus expressing the E? glycoprotein of classical swine fever virus (CSFV) was produced. Engineered with the gX signal sequence of the pseudorabies virus, and transmembrane domain of E2, the E2 protein was expressed as a dimeric form in the cytoplasm of the infected cells. (C) 2001 Elsevier Science B.V. All rights reserved.
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