期刊
BIOTECHNOLOGY LETTERS
卷 23, 期 7, 页码 525-530出版社
SPRINGER
DOI: 10.1023/A:1010374519891
关键词
curdlan; dissolved oxygen; inferential control; optimal control
The pH control was important for curdlan production with Agrobacterium sp. ATCC31750. Specific cell growth rate was the highest at pH 7 and the specific curdlan production rate was at pH 5.5. The pH profiles maximizing curdlan production was changed from pH 7 optimal for cell growth to pH 5.5 optimal for curdlan production after ammonium consumption. The feedback inferential control methods, with easily measurable variables such as NaOH addition for pH control and dissolved oxygen (DO), were also applied. The pH was successfully controlled to follow optimal profiles and the maximal production of curdlan (60 g l(-1) in 120 h) was achieved with feedback optimal control.
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