期刊
DIGESTIVE DISEASES AND SCIENCES
卷 46, 期 4, 页码 836-844出版社
SPRINGER
DOI: 10.1023/A:1010764720524
关键词
oxidized proteins; nitrotyrosine; Helicobacter pylori; cagA; interleukin-8; inducible nitric oxide synthase
In order to study the role of Helicobacter pylori infection in gastric carcinogenesis, we have measured oxidized (carbonyls) and nitrated (nitrotyrosine-containing) proteins as markers for oxidative and nitrative stress in 216 human gastric biopsies using dot and western immunoblots and correlated the results with H. pylori, cagA status, expression of interleukin-8 and inducible nitric oxide synthase (iNOS) mRNAs, and gastric pathology. Higher levels of both oxidized and nitrated proteins were found in patients with either chronic gastritis or duodenal ulcer than in those with normal mucosa. The Levels of modified proteins were significantly higher in inflamed samples infected with H. pylori, especially cagA strains, and in those with expression of interleukin-8 and iNOS mRNAs than in those negative for these parameters. These results indicate that infection with cagA(+) H. pylori induces significant oxidative and nitrative stress in stomach mucosa. contributing to the pathogenesis of H. pylori-associated gastroduodenal diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据