期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 67, 期 4, 页码 1536-1541出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.67.4.1536-1541.2001
关键词
-
资金
- NIGMS NIH HHS [R01 GM022086, GM 22086, R56 GM022086] Funding Source: Medline
The coral-bleaching bacterium Vibrio shiloi biosynthesizes and secretes an extracellular peptide, referred to as toxin P, which inhibits photosynthesis of coral symbiotic algae (zooxanthellae). Toxin P was produced during the stationary phase when the bacterium was grown on peptone or Casamino Acids media at 29 degreesC. Glycerol inhibited the production of toxin P. Toxin P was purified to homogeneity, yielding the following 12-residue peptide: PYPVYAPPPVVP (molecular weight, 1,295.54). The structure of toxin P was confirmed by chemical synthesis. Tn the presence of 12.5 mM NH4Cl, pure natural or synthetic toxin P (10 muM) caused a 64% decrease in the photosynthetic quantum yield of zooxanthellae within 5 min. The inhibition nas proportional to the toxin P concentration. Toxin P bound avidly to zooxanthellae, such that subsequent addition of NK,CI resulted in rapid inhibition of photosynthesis. When zooxanthellae were incubated in the presence of NH,CI and toxin P, there was a rapid decrease in the pH (pH 7.8 to 7.2) of the bulk liquid, suggesting that toxin P facilitates transport of NH, into the cell, It is known that uptake of NH, into cells can destroy the pH gradient and block photosynthesis. This mode of action of toxin P can help explain the mechanism of coral bleaching by V.shiloi.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据