期刊
JOURNAL OF VIROLOGY
卷 75, 期 8, 页码 3740-3752出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.75.8.3740-3752.2001
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资金
- NCI NIH HHS [R37 CA012055, CA12055, R01 CA012055, CA16038, P01 CA016038] Funding Source: Medline
- NIGMS NIH HHS [T32 GM007205, GM07205] Funding Source: Medline
In immunodeficient hosts, Epstein-Barr virus (EBV) often induces extensive B-cell lymphoproliferative disease and lymphoma. Without effective in vitro immune surveillance, B cells infected by the virus readily form immortalized cell lines. In the regression assay, memory T cells inhibit the formation of foci of EBV-transformed B cells that follows recent in vitro infection by EBV. No one has yet addressed which T cell regulates the early proliferative phase of B cells newly infected by EBV. Using new quantitative methods, we analyzed T-tell surveillance of EBV-mediated B-cell proliferation. We found that CD4(+) T cells play a significant role in limiting proliferation of newly infected, activated CD23(+) B cells. In the absence of T cells, EBV-infected CD23(+) B cells divided rapidly during the first 3 weeks after infection. Removal of CD4(+) but not CD8(+) T cells also abrogated immune control. Purified CD4(+) T cells eliminated outgrowth when added to EBV-infected B cells. Thus, unlike the killing of EBV-infected lymphoblastoid cell lines, in which CD8(+) cytolytic T cells play an essential role, prevention of early-phase EBV-induced B-cell proliferation requires CD4(+) effector T cells.
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