期刊
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
卷 47, 期 2, 页码 53-61出版社
MICROBIOL RES FOUNDATION
DOI: 10.2323/jgam.47.53
关键词
cellulase-inducible; chitinase; mycolytic enzyme preparation; Trichoderma viride
Usukizyme, a commercial enzyme preparation from Trichoderma viride, showed multiple chitin-degrading activities. One of these was purified to homogeneity by sequential DEAE Sepharose CL-6B, Q-Sepharose FF, and Sephacryl S-100 HR column chromatographies. The purified enzyme showed optimum activity at pH 3.5 and 50 degrees -55 degreesC and was stable in the pH range of 3.5-6.0 and up to 45 degreesC. It showed higher activity toward chitosan-7B, a 62% deacetylated chitosan, as opposed to highly deacetylated chitosan substrates. Products of degradation of a 1% (w/v) solution of partially deacetylated chitin (PC-100) were purified on CM-Sephadex C-25 and analyzed by HPLC, exo-glycosidase digestion, and nitrous acid deamination. The enzyme was unable to split the GlcN-GlcN linkages in the substrate. It produced mainly (GlcNAc)(2) and (GlcNAc)(3) along with mixed oligosaccharides. When subjected to nitrous acid degradation, some of the mixed oligosaccharides produced mainly 2-deoxyglucitol, implying the presence of GlcN at the reducing end of the oligosaccharides.
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