期刊
BIOMATERIALS
卷 22, 期 8, 页码 865-872出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0142-9612(00)00250-7
关键词
tissue engineering; biodegradable polymers; cell spreading; surface adsorption; poly(L-lysine); RGD
The immobilization of adhesion peptide sequences (such as RGD) at the surfaces of poly(alpha -hydroxyacid)s, including poly(lactic acid) (PLA), is complicated by an absence of functional groups to support covalent attachment. We demonstrate a met hod to overcome this problem, by attaching the peptide to poly(L-lysine) (PLL), which immobilizes the sequence through adsorption at the poly(alpha -hydroxyacid) surface. When coated using a 0.01% w/v solution of PLL-GRGDS, bovine aortic endothelial cells seeded upon the modified PLA showed a marked increase in spreading over unmodified PLA. However, inhibition of the cell-spreading effect occurred when using higher concentrations of PLL-GRGDS, which we attribute to the PLL component. This inhibitory effect can be challenged by increasing the amount of GRGDS attached to each PLL molecule. Potentially, this is a flexible method of surface modification that can engineer many different types of tissue engineering scaffolds with a variety of biomolecules, thus allowing initial cell adhesion to be controlled. (C) 2001 Elsevier Science Ltd. All rights reserved.
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